Koslowsky and Sun awarded NIH GrantYanni Sun, Assistant Professor in Computer Science and Engineering and Donna Koslowsky, Associate Professor in Microbiology and Molecular Genetics, have been funded by the National Institutes of Health for a project entitled "Deep sequence profiling of gRNA transcriptomes in two stages of Trypanosoma brucei".
The objective of this project is to utilize deep sequencing technology to characterize the full guide RNA (gRNA) transcriptomes in the two main life cycle stages of Trypanosoma brucei. Being one of the most striking and ancient examples of small RNA regulation of gene expression, gRNA is responsible for RNA editing in the mitochondria of trypanosomes. High-throughput sequencing will allow the identification of all gRNAs within a population, provide quantitative information on their expression levels and determine the transcriptional structure of the gRNA genes. This project will provide insight into both the developmental regulation of editing and the selective use of alternative guide RNAs.
(Date Posted: 2011-03-07)